HTB-177 NCI-H460 人大细胞肺癌细胞

简要描述：HTB-177 NCI-H460 人大细胞肺癌细胞，ATCC 细胞|细胞系|细胞株|菌种；细胞库管理规范，提供的细胞株背景清楚，提供参考文献和培养条件!

产品型号：
厂商性质：生产厂家
更新时间：2025-09-11
访问量：2620

产品咨询联系我们

产品分类

Product Category

细胞库

细胞原代牛、狗、鸡、鸭、鱼原代细胞羊原代细胞猪原代细胞兔原代细胞小鼠原代细胞大鼠原代细胞人原代细胞培养基原代细胞冻存液 ATCC 人乳腺癌猪正常细胞猪睾丸细胞系人胶质瘤细胞人成骨细胞人永生化肝细胞人肝癌细胞人卵巢癌细胞人结直肠腺癌人经典小细胞肺癌细胞人小细胞肺癌细胞人结直肠腺癌细胞人鼻腔上皮细胞中国仓鼠卵巢悬浮细胞嗜性逆转录病毒包装稳定细胞株小鼠神经细胞瘤人眼脉洛膜黑色素瘤 PT67 逆转录酶病毒包装细胞鸭子胚胎成纤维细胞猪肾细胞系昆虫细胞猫肾细胞恒河猴胚肾细胞仓鼠卵巢细胞负鼠肾细胞仓鼠肺细胞果蝇胚胎细胞家猫肺成纤维样细胞鸡胚细胞豚鼠心肌来源细胞豚鼠肺成纤维样细胞豚鼠皮肤成纤维样细胞狗肾细胞非洲绿猴胚胎肾上皮细胞猴胚胎肾上皮细胞长臂猿淋巴瘤新生牛眼囊成纤维细胞黑化大家鼠肺成纤维样细胞俄勒冈地鼠细胞家兔皮肤成纤维样细胞昆虫卵巢细胞人胸膜瘤细胞非洲绿猴肾细胞 VERO 衍生株（SVP）白化金黄地鼠肺成纤维样细胞黄胸鼠肺成纤维样细胞白鲢尾鳍细胞蝙蝠肺细胞草鱼肾细胞系大黄鱼肾细胞猴肾细胞系犬肾细胞系人结肠直肠腺癌猪肺泡巨噬细胞 PFHSA05（TLL2）人重组特洛德样蛋白因子 PFHSA04 （BMP1）人重组骨诱导因子人乳腺癌细胞结肠癌细胞成纤维细胞人胚肾细胞动物卵巢细胞动物胚胎细胞动物肺细胞肋骨来源细胞成纤维样细胞动物上皮细胞动物肝脏细胞可诱导表达稳定细胞株动物气管细胞动物肾细胞动物巨噬细胞人正常细胞人肿瘤细胞、癌细胞小鼠正常细胞小鼠肿瘤细胞、癌细胞大鼠正常细胞大鼠肿瘤细胞其它动物细胞查看全部产品

详细介绍

HTB-177 NCI-H460 人大细胞肺癌细胞

ATCC® Number: HTB-177™

Designations: NCI-H460 [H460]

Depositors: AF Gazdar, JD Minna

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens (human)

Morphology: epithelial

Source: Organ: lung

Disease: carcinoma; large cell lung cancer

Derived from metastatic site: pleural effusion

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Isolation: Isolation date: 1982

Tumorigenic: Yes

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 11,12

D13S317: 13

D16S539: 9

D5S818: 9,10

D7S820: 9,12

THO1: 9.3

TPOX: 8

vWA: 17

Cytogenetic Analysis: modal numbr = 57; range = 53 to 65.This is a hypotriploid human cell line. The modal chromosome number is 57 although cells with 58 chromosomes occurred with a comparable frequency. The frequency of higher ploidies was 1.7%. Seven marker chromosomes, der(9)t(1;9)(q21;p24), der(9)t(7;9)(p11;p22), t(10q14q), der(16)t(7;16)(q11.23;q22), a small ring (about 1/2 the size of a G chromosome) and two others, were common to all cells. Three other markers were found in some cells only. The markers, t(7;9) and t(7;16) were mostly paired. Normal N9 was absent, and N7 and N16 had only a single copy per cell. Two copies each of the X and the Y were present in all cells.

Isoenzymes: AK-1, 1

ES-D, 1

G6PD, B

GLO-I, 1-2

Me-2, 1

PGM1, 1

PGM3, 1

Gender: male

Comments: The NCI-H460 cell line was derived by A.F. Gazdar and associates in 1982 from the pleural fluid of a patient with large cell cancer of the lung.

The cells express easily detectable p53 mRNA at levels comparable to normal lung tissue, and exhibit no gross structural DNA abnormalities.

The cells stain positively for keratin and vimentin but are negative for neurofilament triplet protein.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Subculturing: Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:8 is recommended

Medium Renewal: Twice per week

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Doubling Time: 23 hrs in medium with serum; 42 to 60 hrs in serum

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

References: 1605: Banks-Schlegel SP, et al. Intermediate filament and cross-linked envelope expression in human lung tumor cell lines. Cancer Res. 45: 1187-1197, 1985. PubMed: 2578876

1806: Takahashi T, et al. p53: A frequent target for genetic abnormalities in lung cancer. Science 246: 491-494, 1989. PubMed: 2554494

22434: Brower M, et al. Growth of cell lines and clinical specimens of human non-small cell lung cancer in a serum-free defined medium. Cancer Res. 46: 798-806, 1986. PubMed: 3940644

32488: Geiger T, et al. Antitumor activity of a PKC-alpha antisense oligonucleotide in combination with standard chemotherapeutic agents against various human tumors transplanted into nude mice. Anticancer Drug Des. 13: 35-45, 1998. PubMed: 9474241

HTB-177 NCI-H460 人大细胞肺癌细胞

留言框

产品：

您的单位：

您的姓名：

联系电话：

常用邮箱：

省份：

详细地址：

补充说明：

验证码：