CRL-9520 SV-HUC-1 人膀胱上皮永生化细胞
| ATCC® Number: | CRL-9520™ | Price: | $417.00 |
| Designations: | SV-HUC-1 |
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| | Depositors: | Wisconsin Alumni Res. Fndn. |
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| | Biosafety Level: | 2 [CELLS CONTAIN ADENOVIRUS ] |
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| | Shipped: | frozen |
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| | Medium & Serum: | See Propagation |
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| | Growth Properties: | adherent |
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| | Organism: | Homo sapiens (human) |
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| | Morphology: | epithelial
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| | Source: | Organ: ureter Tissue: uroepithelium Cell Type: epithelial SV40 immortalizedSV40 transformed |
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| | Cellular Products: | uroepithelial keratins; SV40 T antigen |
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| | Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | | CRL-9520 SV-HUC-1 人膀胱上皮永生化细胞 |
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| | Tumorigenic: | No |
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| | Cytogenetic Analysis: | The chromosome number distribution is bimodal with 50% of the cells near diploid (mode = 44 chromosomes) and 50% tetraploid (mode = 88). Both populations consistently showed seven marker chromosomes: 5p+, del(6)(p11), 9q+, 11p+, 15q-, 19p+ and Xp+. Most cells lacked a normal chromosome 15. Cells in the tetraploid population did not exactly duplicate the diploid population in that most had a 14q/21 translocation involving two chromosomes 14 and two chromosomes 21, while the diploid cells had normal chromosomes 14 and 21 and a 6q/14q translocation. |
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| | Age: | 11 years adult |
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| | Gender: | male |
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| | Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C |
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| | Subculturing: | Protocol: Remove medium, add fresh 0.25% Trypsin, 0.03% EDTA solution and allow the flask to sit at room temperature for 20 minutes. Add fresh medium, aspirate and dispense into new flasks. Subc*tion Ratio: 1:2 to 1:5 Medium Renewal: Every 2 to 3 days |
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| | Preservation: | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
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| | Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2004 recommended serum:ATCC 30-2020 derivative:ATCC CRL-9519 |
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| | References: | 3992: Reznikoff CA, Christian BJ. Human uroepithelial cell. US Patent 4,980,290 dated Dec 25 1990 |
CRL-9520 |
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