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CRL-1576 293 人胚肾细胞
ATCC® Number: CRL-1576™
Designations: 12MBr6
Depositors: JL Caputo
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Cercopithecus aethiops
Morphology: epithelial
CRL-1576 293 人胚肾细胞
Source: Organ: lung
Tissue: bronchus
Disease: normal
Cell Type: epithelial
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Isolation date: July, 1979
Receptors: epidermal growth factor （EGF）
Cytogenetic Analysis: modal number = 62; range = 43 to 64.
The modal chromosome number was found in 78% of the cell population. The rate of polyploidy was 2.4%. Three consistent chromosome markers were identified: M1: del（1）9 ?; M2 del（13） and M3. Chromosome #20 is trisomic and the X chromosome is found in the disomic condition.
Age: 2 to 3 years
Gender: male
Comments: 12MBr6 is an epithelial cell line derived from secondary bronchial tissue of a 2- to 3-year-old normal African green monkey. This line was initiated in June, 1979 at the American Type Culture Collection from tissue explants using penicylinders and a trypsin-versene solution for cell dispersal. These cells contain PAS-positive cytoplasmic inclusions. Epidermal growth factor （EGF） was used to initiate and maintain growth. Post-freeze longevity studies indicate that EGF is an essential component of the culture medium for successful long-term propagation of the cells. Progeny from the 12MBr6 were found to undergo at least 23 additional doublings beyond the frozen state.
Propagation: ATCC complete growth medium: Ham's F-12K Medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and supplemented with 30ng/ml epidermal growth factor and 10% fetal bovine serum
Atmosphere: air, 95%; carbon dioxide （CO2）, 5%
Temperature: 37.0°C
Growth Conditions: The line requires epidermal growth factor as a supplement to the medium.
Subculturing: Protocol: .
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% （w/v） Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
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