CRL-1714 TM3 小鼠gaowan间质细胞的详细介绍
CRL-1714 TM3 小鼠gaowan间质细胞
| ATCC® Number: | CRL-1714™ | Price: | |
| Designations: | TM3 |
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| | Depositors: | JP Mather |
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| | Biosafety Level: | 1 |
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| | Shipped: | frozen |
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| | Medium & Serum: | See Propagation |
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| | Growth Properties: | adherent |
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| | Organism: | Mus musculus （mouse） |
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| | Morphology: | epithelial
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| | Source: | Organ: testis
Disease: normal
Cell Type: Leydig cell; |
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| | Cellular Products: | prostaglandin F2a |
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| | Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | | CRL-1714 |
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| | Receptors: | luteinizing hormone （LH）; epidermal growth factor （EGF）; androgen receptor, positive; estrogen receptor, positive; progesterone receptor, positive |
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| | Tumorigenic: | No |
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| | Age: | 11 to 13 days |
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| | Gender: | male |
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| | Comments: | The TM3 cell line responds to LH with an increase in cAMP production, but does not respond to follicle stimulating hormone （FSH）. The maintenance of responsiveness to LH is dependent upon serum lot. In the presence of LH, the cells are capable of metabolizing cholesterol. Tested and found negative for ectromelia virus （mousepox）. |
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| | Propagation: | ATCC complete growth medium: A 1:1 mixture of Ham's F12 medium and Dulbecco's modified Eagle's medium with 2.5mM L-Glutamine, 0.5mM Sodium Pyruvate, 1.2 g/L Sodium Bicarbonate and 15 mM HEPES, 92.5%; horse serum, 5%; fetal bovine serum, 2.5% |
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| | Subculturing: | Protocol: Remove medium, add fresh 0.25% trypsin-0.53mM EDTA solution, rinse, remove trypsin and let the culture sit at room temperature for 2 to 4 minutes. Add fresh medium, aspirate and dispense into new flasks.
Subc*tion Ratio: A subc*tion ratio of 1:20 to 1:200 is recommended
Medium Renewal: Every 3 to 4 days |
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| | Preservation: | Freeze medium: Complete growth medium, 95%, DMSO, 5%
Storage temperature: liquid nitrogen vapor phase |
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| | References: | 1158: Mather JP. Establishment and characterization of two distinct mouse testicular epithelial cell lines. Biol. Reprod. 23: 243-252, 1980. PubMed: 6774781 1159: Mather JP, et al. Culture of testicular cells in hormone-supplemented serum-free medium. Ann. N.Y. Acad. Sci. 383: 44-68, 1982. PubMed: 7046561 |
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