CRL-1711 SF9 昆虫卵巢细胞

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详细介绍

CRL-1711 SF9 昆虫卵巢细胞 的详细介绍

ATCC^® Number:

CRL-1711™

Price:

$275.00

Designations:	Sf9
Depositors:	G Smith, C Cherry, MD Summers
Biosafety Level:	1
Shipped:	frozen
Medium & Serum:	See Propagation
Growth Properties:	mixed: adherent/suspension
Organism:	Spodoptera frugiperda （fall armyworm）
Morphology:	epithelial
Source:	Organ: ovary
Permits/Forms:	In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Isolation:	Isolation date: 1983
Applications:	transfection host （Roche FuGENE® Transfection Reagents technology from amaxa）
Virus Susceptibility:	Nuclear polyhedrosis virus, Autographa californica St. Louis encephalitis virus
Age:	pupa
Gender:	female
Comments:	This line can be used to replicate baculovirus expression vectors. It is important to use the medium described below.
Propagation:	ATCC complete growth medium: Grace's Insect Medium with L-glutamine and supplemented with: 500 mg/L calcium chloride 2800 mg/L potassium chloride 3330 mg/L lactalbumin hydrolysate 3330 mg/L yeastolate 10% Heat-inactivated fetal bovine serum （previously tested for insect cell culture） Atmosphere: air, 100% Temperature: 28.0°C Growth Conditions: The recommended media are formulated for use without CO2. Omission of the yeastolate or lactalbumin hydrolysate will lead to poor performance by this line.
Subculturing:	Protocol: Gently resuspend cells in the spent culture medium by pipetting across the monolayer or by hitting the flask against the palm of your hand （the latter is only preferable when working with larger flasks）. *Subction Ratio:** A subction ratio of 1:5 or greater is recommended Medium Renewal:* Every 2 to 3 days
Preservation:	Freeze medium: culture medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase
References:	22650: Vaughn JL, et al. The establishment of two cell lines from the insect Spodoptera frugiperda （Lepidoptera; Noctuidae）. In Vitro 13: 213-217, 1977. PubMed: 68913 23136: Zhang PF, et al. Susceptibility of the Sf9 insect cell line to infection with adventitious viruses. Biologicals 22: 205-213, 1994. PubMed: 7811453 23294: Smith GE, et al. Modification and secretion of human interleukin 2 produced in insect cells by a baculovirus expression vector. Proc. Natl. Acad. Sci. USA 82: 8404-8408, 1985. PubMed: 3878519 32359: Hall MR, Gibson W. Cytomegalovirus assemblin: the amino and carboxyl domains of the proteinase form active enzym e when separay cloned and coexpressed in eukaryotic cells. J. Virol. 70: 5395-5404, 1996. PubMed: 8764050 32361: Bakker A, et al. Human T-cell leukemia virus type 2 Rex inhibits pre-mRNA splicing in vitro at an early stage of spliceosome formation. J. Virol. 70: 5511-5518, 1996. PubMed: 8764063 32693: Gibson W, et al. Cytomegalovirus "missing" capsid protein identified as heat-aggregable product of human cytomegalovirus UL46. J. Virol. 70: 7454-7461, 1996. PubMed: 8892863 32860: Filtz TM, et al. Purification and G protein subunit regulation of a phospholipase C-beta from Xenopus laevis oocytes. J. Biol. Chem. 49: 31121-31126, 1996. PubMed: 8940109 32882: Lindorfer MA, et al. G protein gamma subunits with altered prenylation sequences are properly modified when expressed in Sf9 cells. J. Biol. Chem. 271: 18582-18587, 1996. PubMed: 8702508 33023: Bruant SS, et al. N-terminal sequences contained in the Src homology 2 qand 3 domains of p120 GTPase-activating protein are required for full catalytic activity toward ras. J. Biol. Chem. 271: 5195-5199, 1996. PubMed: 8617802 33034: Stewart L, et al. Biochemical and biophysical analyses of recombinant forms of human topoisomerase I. J. Biol. Chem. 271: 7593-7601, 1996. PubMed: 8631793 33143: Kawabe J, et al. Soluble adenylyl cyclase from Spodoptera frugiperda （Sf9） cells. J. Biol. Chem. 271: 20132-20137, 1996. PubMed: 8702736 33160: Yasuda H, et al. Role of the prenyl group on the G protein gamma subunit in coupling trimeric G proteins to A1 adenosine receptors. J. Biol. Chem. 271: 18588-18595, 1996. PubMed: 8702509

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